Setting the Standard: Performance Requirements for Fluorescence Imaging

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Date

June 12, 2026

Fluorescence imaging systems vary widely in complexity and cost, from simple plastic lens assemblies to precision-engineered immersion microscope objectives. Understanding the performance requirements up front is essential to building a system that meets the needs of the application without unnecessary complexity.

In this session, Ben Masella, Ph.D., Principal Optical Engineer at Optikos, breaks down the performance specifications that matter most, including field‑of‑view, numerical aperture, sensitivity, and resolution, and shows how these parameters inform system architecture and design decisions.

Watch the Webinar

What You’ll Learn

Viewers will walk away with a practical understanding of:

• The relationship between field‑of‑view, numerical aperture, sensitivity, and resolution
• Ways these parameters influence design direction and system complexity
• Guidelines for determining whether a requirement is properly defined or over‑specified
• How performance requirements shape overall imaging system architecture
• Key considerations for achieving application‑appropriate fluorescence imaging performance

Turn Your Imaging Challenges into Real‑World Solutions. Speak directly with our engineering & applications team at sales@optikos.com.

About Optikos

Optikos delivers innovative optical engineering, manufacturing and metrology imaging system solutions for space, defense, medical, and semiconductor applications. Our engineering teams develop systems that push the boundaries of optical performance and reliability.


This webinar originally aired as part of the BioPhotonics Fluorescence Microscopy Summit hosted by Photonics Media. Optikos is pleased to offer the full on‑demand recording, along with downloadable resources for continued learning.

About this Presenter

Ben Masella, Ph.D. fluorescence imaging performance requirements

Ben Masella, PhD.

Ben Masella, Ph.D. is a Principal Optical Engineer at Optikos Corporation, where he leads optical design, tolerancing, and advanced imaging system development for metrology and instrumentation applications. His background includes extensive experience in infrared optics, adaptive optics retinal imaging, and sequential/non‑sequential modeling using Code V, Zemax, and FRED. Ben holds a doctorate in optics from the University of Rochester.